Theoretical classes will cover the following topics. Cell and tissue culture (5 h): principles of cell and tissue culture; media for cell cultivation; cell growth and metabolism; cell characterization; cryopreservation; contaminants; scale-up; cell-based secretome: exosomes. Stem cells and derivatives for regenerative medicine (9 h): stem-cell properties; multipotent stem cells; stem cell niches and microenvironmental cues; pluripotent stem cells: embryonic and induced pluripotent stem cells; cell reprogramming; cell modulation by CRISPR; stem cell processing: cell isolation, purification, expansion and controlled differentiation; microtechnologies and biomaterials for stem cell bioengineering. Stem cells for disease modeling (8 h): lab- on-a-chip, high-throughput screening, high content analyses. Cell-based therapies (8 h). Laboratory classes (20 h). cell culture; gene expression analyses; immunocytochemistry; flow cytometry analyses.
Bibliography of reference
Palsson, B.Ø. and Bhatia, S.N., Tissue Engineering, Pearson Prentice Hall Bioengineering, 2004 Vunjak-Novakovic, G. and Freshney, R., Culture of Cells for Tissue Engineering, Wiley, 2006 Atala, A., Lanza, R., et al, Principles of Regenerative Medicine, Academic Press, 2007 Schaffer, D., Bronzino J.D., Peterson, D.R., Stem Cell Engineering, Principles and Practices, CRC Press, 2013